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An improved no-moving-parts video-rate confocal microscope
Affiliation:1. State Key Laboratory of Petroleum Resources and Prospecting, CNPC Key Laboratory of Geophysical Exploration, China University of Petroleum, Beijing, People''s Republic of China;2. Bureau of Economic Geology, John A. and Katherine G. Jackson School of Geosciences, The University of Texas at Austin, University Station, Box X, Austin, TX 78713-8924, USA;3. CNOOC Research Institute, Taiyanggongnanjie #6, Chaoyang District, Beijing 100028, China;4. SINOPEC Exploration and Production Research Institute, Beijing, China;1. School of Electronics Engineering, IT College, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea;2. Research Center for Neurosurgical Robotic System, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea;1. Pattern Recognition Lab, Department of Computer Science, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany;2. Department Artificial Intelligence in Biomedical Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany;3. Technische Hochschule Ingolstadt, Ingolstadt, Germany;4. Department of Maxillofacial Surgery, Friedrich-Alexander-Universität Erlangen–Nürnberg, University Hospital, Erlangen, Germany;5. Department of Otorhinolaryngology, Head and Neck Surgery, University of Erlangen–, Nürnberg, Germany;6. Department of Otorhinolaryngology, Plastic Head and Neck Surgery, Rheinische Westfälische Technische Hochschule Aachen, University Hospital, Aachen, Germany
Abstract:Several years ago our research program developed a video-rate confocal microscope with no moving parts, based on synchronizing and aligning the scan of an image dissector tube (IDT) with the light returning from a microscope stage that has been acousto-optically scanned by a laser beam. Improvements on the original system have recently been completed. The laser power has been substantially increased and the laser scan is now brought into the Nikon Diaphot inverted microscope through the epi-illumination port. Aberrations in the scanned beam have been reduced by performing the beam shaping required by the acousto-optic deflectors using prisms instead of cylindrical lenses. The IDT is located at the side camera output port at the end of a simple, efficient light path. The new system is described in detail and results obtained using the microscope in reflection and fluorescence mode are presented.
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