Micronucleus test with ethyl methanesulfonate in mouse peripheral blood reticulocytes stained supravitally using acridine orange-coated slides |
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| Institution: | 1. Department of Radiation Oncology, University of Arizona Health Sciences, Tucson, Arizona;2. Department of Medicine, University of Arizona Health Sciences, Tucson, Arizona;3. Department of Medical Imaging, University of Arizona Health Sciences, Tucson, Arizona;4. Department of Radiology and the Translational Imaging Center, Houston Methodist Research Institute, Houston, Texas;5. Department of Cell and Molecular Medicine, University of Arizona Health Sciences, Tucson, Arizona;1. Cytokinetics, South San Francisco, CA, 94080, United States of America;2. Abbvie, North Chicago, IL 60064, United States of America;3. Ncardia, Leiden 2333 BD, the Netherlands;4. Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC, the Netherlands;5. Charles River Laboratories, Laval, QC H7V 4B3, Canada;6. Cardiovascular Division, King''s College London, Rayne Institute, St Thomas'' Hospital, London SE17EH, UK;1. Division of Cardiology, Department of Medicine, USA;2. Department of Genome Sciences, USA;3. Institute for Stem Cell and Regenerative Medicine, USA;4. Center for Cardiovascular Biology, USA;5. Department of Pathology, University of Washington, Seattle, WA, USA |
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| Abstract: | A new method for the micronucleus test using peripheral blood reticulocytes stained supravitally using acridine orange-coated slides was evaluated in male CD-1 mice treated with ethyl methanesulfonate (EMS) at doses of 100, 200, 300, and 400 mg/kg. Peripheral blood samples were taken 0, 24, 48, 72, and 96 h after treatment from each mouse without killing. The frequencies of micronucleated reticulocytes increased dose-dependently with the peak at 48 h after treatment. These results indicate that, at least for EMS, the new method used here can be an alternative to the conventional method using bone marrow polychromatic erythrocytes. |
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