Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate |
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| Authors: | Stanislav Obruca Ivana Marova Ondrej Snajdar Ludmila Mravcova Zdenek Svoboda |
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| Affiliation: | (1) Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;(2) Faculty of Chemistry, Department of Chemistry and Technology of Environmental Protection, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;(3) Research Institute of Brewing and Malting, Malting Institute Brno, Mostecka 7, 614 00 Brno, Czech Republic |
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| Abstract: | Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l−1, respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l−1 h−1, respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer. |
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