Luciferase-dependent growth of cytochrome-deficient Vibrio harveyi |
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Authors: | John C. Makemson J.Woodland Hastings |
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Affiliation: | Department of Biological Sciences, Florida International University, Tamiami Trail, Miami, FL, 33199, U.S.A.;Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA, U.S.A. |
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Abstract: | Abstract The presence of 10−6 M human serum transferrin (TF) in a minimal medium retarded the growth of Vibrio harveyi and inhibited the synthesis of cytochromes and stimulated the development of bioluminescence. The addition of 10−3 M arginine to the TF medium further stimulated bioluminescence and increased the growth rate of the bacteria. These data suggest that luciferase, functioning as a terminal oxidase, supported the growth of such cytochrome-deficient bacteria. |
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Keywords: | Cytochrome-deficient luminous bacteria transferrin |
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