Isolation and purification of calcium and magnesium dependent endonuclease from rat liver nuclei |
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Authors: | R Ishida H Akiyoshi T Takahashi |
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Institution: | Laboratory of Biochemistry Aichi Cancer Center Research Institute Chikusa-ku, Nagoya, Japan |
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Abstract: | An endonuclease associated with rat liver chromatin was extracted with 0.6 M NaCl and purified by ammonium sulfate fractionation and Sephadex G-100 gel filtration. The enzyme produces single strand scissions on native DNA at neutral pH in the presence of 1 mM CaCl2 and 5 mM MgCl2. Alkali-denatured DNA was not nicked by the enzyme. Omission of Ca2+ reduced the enzyme activity to about one seventh. Without Ca2+, however, Mn2+ was more effective than Mg2+. The molecular weight of the enzyme is about 27,000. |
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