Development of a Monoclonal Antibody Detection Assay for Species-Specific Identification of Abalone |
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Authors: | Andreas L Lopata Thomas Luijx Bartha Fenemore Neville A Sweijd Peter A Cook |
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Institution: | (1) Department of Immunology, Allergology Unit, Groote Schuur Hospital, Observatory 7925, Cape Town, South Africa, ZM;(2) Laboratory for Vaccine Research, National Institute for Public Health and the Environment (RIVM), PO Box 1,370 BA Bilthoven, The Netherlands, NL;(3) Department of Zoology, University of Cape Town, Private Bag Rondebosch 7701, Cape Town, South Africa, ZM |
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Abstract: | Species identification based on biochemical and molecular techniques has a broad range of applications. These include compliance
enforcement, the management and conservation of marine organisms, and commercial quality control. Abalone poaching worldwide
and illegal trade in abalone products have increased mainly because of the attractive prices obtained and caused a sharp decline
in stocks. Alleged poachers have been acquitted because of lack of evidence to correctly identify species. Therefore, a robust
method is required that would identify tissue of abalone origin to species level. The aim of this study was to develop immunologic
techniques, using monoclonal and polyclonal antibodies, to identify 10 different abalone species and subspecies from South
Africa, the United States, Australia, and Japan. The combination of 3 developed monoclonal antibodies to South African abalone
(Haliotis midae) enabled differentiation between most of the 10 species including the subspecies H. diversicolor supertexta
and H. diversicolor diversicolor. In a novel approach, using antibodies of patients with allergy to abalone, the differentiation
of additional subspecies, H. discus discus and H. discus hannai, was possible. A field-based immunoassay was developed to
identify confiscated tissue of abalone origin. |
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