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Improved system for construction and analysis of single-copy beta-galactosidase operon fusions in Yersinia enterocolitica
Authors:Maxson Michelle E  Darwin Andrew J
Institution:Department of Microbiology MSB 228, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
Abstract:We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous beta-galactosidase activity.
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