Synergism of Trichoderma reesei cellulases while degrading different celluloses |
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| Authors: | Bernd Nidetzky Marianne Hayn Ricardo Macarron Walter Steiner |
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| Institution: | (1) Institute of Biotechnology, Technical University of Graz, Petersgasse 12, A- 8010 Graz, Austria;(2) Institute of Biochemistry, University of Graz, Schubertstra e 1, A-8010 Graz, Austria;(3) SmithKlineBeecham Pharmaceuticals, Parque Tecnologico de Madrid, 28760 Tres Cantos (Madrid), Spain |
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| Abstract: | Summary The synergistic action of purified cellulases from Trichoderma reesei in hydrolysis of cellulose decreased with increasing substrate concentration, depended strongly on the the type of cellulose used, and was maximal on crystalline cellulose. Contrarily, the activity of the individual cellulases was highest on amorphous cellulose. The binary combinations CBH I/EG III and CBH I/CBH II exhibited the greatest degree of synergism on crystalline cellulose. |
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