A computer program for determining the size of DNA restriction fragments |
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| Authors: | R G Duggleby H Kinns J I Rood |
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| Affiliation: | Department of Biochemistry, John Curtin School of Medical Research, Australian National University, Canberra, Australia, 2601 |
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| Abstract: | A computer program has been developed for determining the sizes of DNA restriction fragments from their electrophoretic mobilities. A parabola is fitted to the mobilities of a set of standard fragments of known sizes and the sizes of the unknown fragments are then calculated from the fitted curve. This procedure is shown to yield estimated sizes which are accurate to within a few percentage, as judged by experiments with fragments obtained by digestion of pBR322 with the restriction endonuclease HaeIII. The program, which is written in BASIC, is simple to use and is very much faster than the graphical method that it replaces. |
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| Keywords: | Present address and address for reprints and correspondence: Department of Biochemistry University of Queensland St. Lucia 4067 Australia. |
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