Expression of c-Myc is related to host cell death following <Emphasis Type="Italic">Salmonella typhimurium</Emphasis> infection in macrophage |
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Authors: | Jihyoun?Seong Hong?Hua?Piao Phil?Yeoul?Ryu Youn?Uck?Kim Hyon?E?Choy Email author" target="_blank">Yeongjin?HongEmail author |
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Institution: | (1) Clinical Vaccine R&D Center, Chonnam National University Medical School, Gwangju, 501-746, Republic of Korea;(2) Department of Microbiology, Chonnam National University Medical School, Gwangju, 501-746, Republic of Korea;(3) Department of Biomedical Sciences, Sun Moon University, A-San, 336-708, Republic of Korea |
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Abstract: | It has been known that ornithine decarboxylase (ODC) induced by the binding of c-Myc to odc gene is closely linked to cell death. Here, we investigated the relationship between their expressions and cell death in
macrophage cells following treatment with Salmonella typhimurium or lipopolysaccharide (LPS). ODC expression was increased by bacteria or LPS and repressed by inhibitors against mitogen-activated
protein kinases (MAPKs) in Toll-like receptor 4 (TLR4) signaling pathway. In contrast, c-Myc protein level was increased after
treatment with bacteria, but not by treatment with LPS or heat-killed bacteria although both bacteria and LPS increased the
levels of c-myc mRNA to a similar extent. c-Myc protein level is dependent upon bacterial invasion because treatment with cytochalasin D
(CCD), inhibitors of endocytosis, decreased c-Myc protein level. The cell death induced by bacteria was significantly decreased
after treatment of CCD or c-Myc inhibitor, indicating that cell death by S. typhimurium infection is related to c-Myc, but not ODC. Consistent with this conclusion, treatment with bacteria mutated to host invasion
did not increase c-Myc protein level and cell death rate. Taken together, it is suggested that induction of c-Myc by live
bacterial infection is directly related to host cell death. |
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Keywords: | Salmonella typhimurium c-Myc infection ornithine decarboxylase macrophage cell death |
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