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Isolation of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting |
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| Authors: | A I Faerman A V Chervonski? T A Chipysheva V I Gel'shte?n A Iu Koliada |
| Abstract: | Monoclonal anti-peroxidase antibodies (McAb) were generated by means of hybridization of BALB/c immune splenocytes with X-63.653 cells. Peroxidase-antiperoxidase (PAP) staining of fibroblast cultures with murine McAb against fibronectin was used for the detection of positive cultures. McAb AP-FC-2B4 were found to be highly efficient in different immunoenzyme tests, e.g., immunochemistry and immunoblotting. PAP method was used for histological diagnosis of invasive human breast cancer. |
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