Acid-active neuraminidases in the growht media from cultures of pathogenic Naegleria fowleri and in sonicates of rabbit alveolar macrophages |
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| Institution: | 1. Department of Organismal Biology, Program in Systematic Biology, Uppsala University, Uppsala, Sweden;2. Science for Life Laboratory, Uppsala University, Uppsala, Sweden;3. Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada;4. Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, NS, Canada;5. Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA;6. Institute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS, USA;7. Department of Biology, Dalhousie University, Halifax, NS, Canada |
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| Abstract: | Using bovine mucin and isolated human myelin as source of sialic acid, we demonstrate the presence of neuraminidase activities in the growth media of pathogenic, but not nonpathogenic, Naegleria sp. and in sonicates of rabbit alveolar macrophages. Neuraminidase activity was maximal at pH 4.5 and 5.0, and the specific activity for sialic release was up to 13-fold greater with mucin that with human myelin. Activity in the growth media from cultures of pathogenic Naegleria fowleri was ion-independent, while that of macrophage sonicates required divalent cation; optimal activity was noted with 2.5 mM Zn2, while Mg2+ and Mn2+ supported activity to a lesser extent. Such acid-active neuraminidases may contribute to the reported glycolipid alterations associated with the demyelinating diseases. |
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