Imparied formylation and uptake of tetrahydrofolate by rat small gut following cobalamin inactivation |
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| Institution: | 1. Department of Seafood Processing Technology, Faculty of Fisheries, Cukurova University, 01330, Adana, Turkey;2. University Department of Marine Studies, University of Split, Ruđera Boškovića 37, 21000, Split, Croatia;3. Department of Animal Product Technology, Faculty of Food Technology, University of Agriculture in Krakow, Ul, Balicka 122, 30-149, Kraków, Poland;4. Nofima AS, Norwegian Inst Food Fisheries & Aquaculture Res, N-9291, Tromso, Norway;5. Université Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-63370, Lempdes, France;6. Post Graduate Institute of Post-harvest Management (Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth), Roha, Raigad, Maharashtra State, India;7. Mumbai Research Centre of ICAR-CIFT, CIDCO Admin Building, Vashi, Navi Mumbai, 400 703, India;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. National R&D Center of Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China;3. Engineering Research Center of Freshwater Fish High-value Utilization of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China;1. National Engineering Research Center of Seafood, Dalian 116034, People’s Republic of China;2. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, People’s Republic of China;3. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, People’s Republic of China;1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;2. Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China;1. School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China;2. National Engineering Research Center of Seafood, Dalian, 116034, China;3. State Key Laboratory of Marine Food Processing and Safety Control, Dalian, 116034, China;4. College of Biological System Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China |
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| Abstract: | The effect of inactivation of cobalamin by N2O in the intestinal absorption of folate was studied using rat everted gut sacs. Further, in view of uncertainties about the presence of methionine synthetase in gut 1], this enzyme was measured. Everted gut sacs were incubated with 2-14C]tetrahydrofolate, and the subsequent appearance of labelled formyl- and methyl14C]tetrahydrofolate in everted segments of small intestine of rats was studied. Considerable methionine synthetase activity was present in washed everted gut sacs but not in gut segments in the absence of such treatment. Methionine synthetase activity declined after exposure to N2O, which oxidizes and inactivates cob(I)alamin. Folate uptake by gut sacs was not affected by 24 h exposure of the animals to N2O but fell significantly after 7 days exposure. There was a significant fall in the amount of formlytetrahydrofolate formed after cobalamin inactivation and this was reversed by supplying either methionine, methylthioadenosine or sodium formate. Serine had no effect. The data support the hypothesis that methionine and methylthioadenosine act by supplying single carbon units at the formate level of oxidation. |
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