Site-directed integration of transgenes: transposons revisited using DNA-binding-domain technologies |
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Authors: | Marie-Véronique Demattei Xavier Thomas Elodie Carnus Corinne Augé-Gouillou Sylvaine Renault |
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Institution: | 1.Université Fran?ois Rabelais de Tours, GICC,Tours,France;2.CNRS, UMR 6239,Tours,France;3.CHRU de Tours, UFR des Sciences & Techniques, Parc de Grandmont,Tours,France;4.Université Fran?ois Rabelais, UFR des Sciences & Techniques, Parc de Grandmont,Tours,France |
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Abstract: | In the last 20 years, tools derived from DNA transposons have made major contributions to genetic studies from gene delivery
to gene discovery. Various complementary and fairly ubiquitous DNA vehicles have been developed. Although many transposons
are efficient DNA vehicles, they appear to have limited ability to target specific sequences, since all that is required at
the integration locus is the presence of a short 2- to 4-bp sequence. Consequently, insertions mediated by transposon-based
vectors occur somewhat randomly. In the past 5 years, strategies have emerged to enhance the site-specificity of transposon-based
vectors, and to avoid random integrations. The first proposes that new target site specificity could be grafted onto a transposase
by adding a new DNA-binding domain. Alternative strategies consist of indirectly targeting either the transposase or the transposon
to a chosen genomic locus. The most important information available about each strategy are presented, and limitations and
future prospects are discussed. |
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