Overproduction, purification and crystallization of Bacillus cereus oligo-1,6-glucosidase |
| |
| Authors: | K Watanabe K Kitamura Y Hata Y Katsube Y Suzuki |
| |
| Affiliation: | Department of Agricultural Chemistry, Kyoto Prefectural University, Japan. |
| |
| Abstract: | The gene coding for oligo-1,6-glucosidase from Bacillus cereus ATCC7064 has been overexpressed in Escherichia coli MV1184 cells under the control of the lac promoter in the genetically engineered plasmid pBCE4-2. Oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25 degrees C by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. The crystals have the shape of hexagonal bipyramids and belong to the space group P6(2) or P6(4) with lattice constants of a = b = 106.1 A, c = 120.0 A and gamma = 120 degrees. |
| |
| Keywords: | |
|
|
