Effect of gamma irradiation on microbial load,aflatoxins and phytochemicals present in Trigonella foenum-graecum |
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| Authors: | Gupta Prakash C. Bajpai Vivek Mishra V. Saxena R. K. Singh Surendra |
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| Affiliation: | (1) Microbiology Laboratory, Homoeopathic Pharmacopeia Laboratory, Ghaziabad, 201002, India;(2) Microbiology Department, Bundelkhand University, Jhansi, 284128, India;(3) Department of Biotechnology, Mody Institute of Technology & Science, Laxmangarh, Sikar, 332311, India;(4) Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India |
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| Abstract: | The effects of gamma irradiation on microbial load, total aflatoxins and phytoconstituents content of Trigonella foenum-graecum have been studied. Gamma irradiation at a dose of 2.5 kGy resulted in 2 log reduction of the total aerobic microbial count. A complete sterilization was, however, observed at 10 kGy. The total aflatoxin level decreased gradually with increase in gamma irradiation dose as compared to its un-irradiated counterparts, whereas the high performance liquid chromatography (HPLC) profile showed no change in the levels of phytochemicals up to the gamma irradiation dose of 10 kGy. HPLC profiles, however, differed in peak areas, and retention times of the components. These results suggest that gamma irradiation at a dose of 5.0 kGy was very effective for microbial decontamination because it did not adversely affect the active components of T. foenum-graecum. |
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