Structure of the antigenic O-polysaccharide of the lipopolysaccharide produced by Salmonella eimsbuttel |
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Authors: | J L Di Fabio M B Perry J R Brisson |
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Institution: | Division of Biological Sciences, National Research Council, Ottawa, Ont., Canada. |
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Abstract: | The smooth lipopolysaccharide produced by Salmonella eimsbuttel, which had the O:6, O:7, and O:14 antigenic factors defined in the Kauffmann-White classification, was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, composition analysis, methylation, periodate oxidation, deamination, and 1H and 13C nuclear magnetic resonance studies to contain a high molecular weight O-chain polysaccharide composed of D-mannose (four parts), D-glucose (one part), and 2-acetamido-2-deoxy-D-glucose (one part). It was a branched polymer of a repeating hexasaccharide unit having the structure (formula; see text). |
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