Mitochondrial nucleoside diphosphate kinase: Mode of interaction with the outer mitochondrial membrane and proportion of catalytic activity functionally coupled to oxidative phosphorylation |
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Authors: | T Yu Lipskaya V V Voinova |
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Institution: | (1) Department of Biochemistry, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia |
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Abstract: | In the present study, we found that ionic interactions are not essential for the binding of nucleoside diphosphate kinase
of liver mitochondria outer compartment to outer mitochondrial membrane and that the proportion of the enzyme activity involved
in functional coupling with oxidative phosphorylation (we demonstrated the existence of functional coupling earlier) is only
17%. Additional evidence was obtained that functionally coupled activity of nucleoside diphosphate kinase is associated with
the outer surface of mitochondria. Dextran (10%) did not increase functional coupling. The physological importance of these
effects is discussed.
Published in Russian in Biokhimiya, 2008, Vol. 73, No. 3, pp. 395–407. |
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Keywords: | nucleoside diphosphate kinase mitochondria outer compartment localization oxidative phosphorylation functional coupling |
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