The effect of Ca2+ on the oxidation of exogenous NADH by rat liver mitochondria. |
| |
| Authors: | P Gazzotti |
| |
| Institution: | Department of Biochemistry, Swiss Federal Institute of Technology (ETH) Zurich, Switzerland |
| |
| Abstract: | The respiratory rate of rat liver mitochondria in the presence of NADH as exogenous substrate is enhanced by the addition of CaCl2 (> 50 μM) when inorganic phosphate is present in the medium. The Ca-induced oxidation of NADH is inhibited by rotenone but is not affected by uncoupling agents. EDTA, which does not reverse the swelling of mitochondria which occurs in the presence of Ca2+ and phosphate, is able to inhibit reversibly the Ca-stimulated NADH oxidation. A stimulation of the rate of oxidation of NADH by Ca2+ is also observed in mitochondria partially swollen in a hypotonic medium. |
| |
| Keywords: | EDTA ethylendiaminetetracetic acid HEPES N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid DNP 2 4 dinitrophenol SMP submitochondrial particles |
| 本文献已被 ScienceDirect 等数据库收录！ |
