Identification of two quantitative trait loci involved in antibody production on mouse chromosome 8 |
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Authors: | A Puel Jean-Claude Mevel Yolande Bouthillier Claude Decreusefond Wolf H Fridman Nicole Feingold D Mouton |
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Institution: | (1) Institut National de la Santé et de la Recherche Médicale Unité 255, Institut Curie, 26 rue d’Ulm, F-75231 Paris Cedex 05, France, FR;(2) Institut National de la Santé et de la Recherche Médicale Unité 155, Université Paris VII, F-75251 Paris Cedex 05, France, FR |
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Abstract: | Several quantitative trait loci (QTLs) contributing to the extreme phenotypes of the selected high (H) and low (L) antibody-responder
lines of mice were mapped on distinct chromosomes. Successive backcrosses were bred to reduce the length of the QTL-bearing
segment detected on chromosome 8 and to produce congenic lines to test gene effect independently of the other QTLs. An increase
in antibody responses was repeatedly found to be associated with inheritance of the H-line allele at two markers separated
by 30 cM on that chromosome. In the successive backcrosses, background and unlinked involved genes of H-line origin were progressively
eliminated; however, unexpected within-progeny variations persisted in the third and even fourth backcross. Nevertheless,
the presence of two QTLs within the considered interval was definitely demonstrated in distinct progenies of the fourth backcross
which separately inherited one of the two gene-marker H-line alleles. The previously identified chromosome 8 segment therefore
contains at least two QTLs involved in antibody responsiveness.
Received: 19 August 1997 / Revised: 9 October 1997 |
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Keywords: | Biozzi mice Mouse chromosome 8 QTL Antibody production Microsatellite |
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