重楼属植物的免疫血清学研究

以海南重楼(Paris dunniana),花叶重楼(P.marmorata),多叶重楼(P.polyphylla),凌云重楼(P.cronquistii),五指莲(P.axialis),滇重楼(Paris polyphylla var.yunnanensis)等六种重楼属植物的根茎为材料,提取分离其球蛋白组份作为抗原,免疫家兔得到相应的抗血清。通过免疫双扩散,聚丙烯酰胺凝胶电泳、免疫电泳、免疫吸收试验以及酶标免疫等方法,研究了重楼属上述六个种和变种的血清学行为以及他们之间的相互关系。在此基础上,用不加权算术平均对群法(UPGMA)经成聚运算,得到基于平均吸收相似系数(Sabs.mean.)和Jaccard's结合系数的两种表相图,这两种表相图的结果是一致的。花叶重楼与多叶重楼有较大的血清反应相似性,五指莲与滇重楼有最大的血清反应相似性。多型种P.polyphylla在形态上的较大变异与血清反应分析的结果是一致的,而且血清反应相似性分析支持了从形态分析得出的假设:Paris dunniana在重楼属的系统发生中是一比较原始的种。

SEROLOGICAL INVESTIGATION OF SOME TAXA OF PARIS GENUS

The immunological relationships of six taxa of Paris genus were investigated using their root stock globulin components as the antigens and employing poly-acrylamine gel electrophoresis (PAGE), immunodiffusion, imm-unoclectrophoresis (IEP), IEP combined with absorption technique and enzyme-linked immunosorbent assay (ELISA). The dendrograms of immunological relationship calculated by Mean Absorption Similarity Coefficient (Sabs, mean) and Jaccard's coefficient were generated. The results obtained using above two coeffecients were both the same. The result based on ELISA also matched the one by Jaccard's and Sabs, mean well. Serological data supported the assumption that the P. dunniana section is a primitive group in the phylogeny of Paris.