金葡菌L型感染致小鼠肿瘤中CyclinD1、CDK4和P16的表达及意义

目的探讨金黄色葡萄球菌(金葡菌)L型感染C57小鼠致瘤后,CyclinD1、CDK4和P16在小鼠肿瘤及癌前病变中的表达及相关性研究。方法动物实验观察发现金葡菌L型感染90只C57小鼠后11.1%(10/90)发生肿瘤;14.4%(13/90)引起癌前病变。革兰染色、免疫组化染色,检测小鼠肿瘤和癌前病变中金葡菌L型感染率和CyclinD1、CDK4和P16蛋白的阳性表达。结果 10只小鼠肿瘤及13只小鼠癌前病变中金葡菌L型检出率与正常对照组比较差异有明显统计学意义(P0.01)。CyclinD1、CDK4和P16蛋白的阳性表达与正常对照组比较差异有统计学意义(P0.01～0.05),呈正相关。结论金葡菌L型感染可能与CyclinD1、CDK4和P16蛋白在小鼠肿瘤发生和发展中有协同作用。

The expression and significance of CyclinD1, CDK4 and P16 in mouse tumor caused by Staphylococcus aurues L-form infection

Objective To study the expressions of apoptosis inhibitor proteins CyclinD1,CDK4 and P16 in the mouse tumors caused by Staphylococcus aureus（S.aureus） L-form infection and in precancerous lesions,and explore their relationship.Method 90 C57 mice were infected with Staphylococcus aureus L-forms.Gram staining and immunohistochemical methods were used to determine the infection rates of Staphylococcus aureus L-form and the expressions of CyclinD1,CDK4 and P16 proteins in mice tumors and precancerous lesions.Result 11.1%（10 /90） of the mice had developed tumor and 14.4%（13 /90） had precancerous lesions.The detection rates of Staphylococcus aureus L-type in the 10 mouse tumors and 13 precancerous lesions were significantly different from those of the normal control group（P 0.01）;The positive expressions of CyclinD1,CDK4 and P16 proteins were significantly different from those of normal control group（P 0.01-0.05）.There was a positive correlation between the two indicators.Conclusion There may be a synergistic relation between L-type Staphylococcus aureus infection and the CyclinD1,CDK4 and P16 proteins in mouse tumor,which plays an important role in the incidence and development of tumor.