Isolation of an Infectious Ribonucleoprotein from Vesicular Stomatitis Virus Containing an Active RNA Transcriptase |
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| Authors: | J. F. Szilágyi and L. Uryvayev |
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| Affiliation: | Medical Research Council Virology Unit, Institute of Virology, University of Glasgow, Scotland |
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| Abstract: | A ribonucleoprotein complex (TNP) containing an active RNA polymerase was isolated from purified vesicular stomatitis virus particles. The TNP sedimented through a sucrose gradient as a single band and appeared under the electron microscope as discrete long filaments in a spiral configuration. TNP contained one major and two minor polypeptides, but not the polypeptides associated with the outer coat of vesicular stomatitis virus. BHK-21 clone 13 cells could be infected with TNP, yielding infectious virus particles. |
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