Abstract: | The acetylation of both amino groups on sulphanilaniide hasbeen demon strated by paper chromatography of sap expressedfrom broad beans treated with suiphanilamide through the roots.Deacetylation of N1- and N4 mides occurred in the leaves oftreated plants and the N4 compound was also acetylated to yieldthe N1: N4-diacetylsulphanilamide. About 30 per cent. of the suiphanilamide entering broad-beanroots was acetylated in the roots, the maximum proportion ofthe N4-acetyl compound being reached after 7 days. Deacetylationis a more limited process but reaches a steady state after thesame time. Low levels of acetylation and deacetylation wererecorded in wheat. The amounts of suiphanilamide and N4 entering and accumulatingin the various tissues were calculated on a water-uptake basisarid these data show that the main site of acetylation is inthe roots and that deacetyla tion occurs to a limited extentin the roots but predominates in stems and leaves. |