重组蛋白质在酵母表达体系中的高效表达策略

酵母由于其本身的一些优良特性和容易操作性，可以高水平表达重组蛋白，近年来已经有多个酵母表达的蛋白质多肽类药物上市。作为宿主的酵母最常用的是毕赤氏酵母和酿酒酵母。本文对酵母的一般特性、酵母表达操作、密码子、载体和表达策略、两种不同酵母表达系统的特点等进行了论述，供研究者进行酵母高效表达体系的选择与操作参考。

The yeast expression system for recombinant protein

Quite a few peptide remedies produced by the yeast expression come into the market recently, because the yeast expression system easily handled with excellent characteristic and producing recombinant protein with high yield. The methylotrophic yeast P. pastoris and S. cerevisiae are in common use as the host for expression of heterologous protein. The traits of both expression systems are compared ; the related coden, vector, and promoter are discussed ; the advanced protocol of operation and the strategy for optimization are surveyed in this article.