Region- and cell type-selective expression of the evolutionarily conserved Nolz-1/zfp503 gene in the developing mouse hindbrain |
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Authors: | Chang Sunny Li-Yun Yan Yu-Ting Shi Yan-Ling Liu Ya-Chi Takahashi Hiroshi Liu Fu-Chin |
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Institution: | aInstitute of Neuroscience, National Yang-Ming University, Taipei 112, Taiwan, ROC;bInstitute of Molecular Systems Biomedicine, China Medical University, Taichung 404, Taiwan, ROC;cInstitute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, ROC;dDepartment of Neurology, National Hospital Organization, Tottori Medical Center, Tottori 689-0203, Japan |
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Abstract: | Nolz-1/Zfp503, a zinc finger-containing gene, is a mammalian member of the SP1-related nocA/elb/tlp-1 gene family. Previous studies have shown that Nolz-1 homologs are important for patterning the rhombomeres in zebrafish hindbrain. We therefore studied the expression pattern of Nolz-1 in the developing mouse hindbrain. Nolz-1 mRNA expression was detected in the prospective rhombomere 3, 5 and caudal regions as early as E8.75. After E11.5, Nolz-1-positive cells were organized as distinct cell clusters, and they were largely non-overlapped with either Pax2-positive or Phox2b-positive domains. Most interestingly, we found that Nolz-1 was specifically expressed by Phox2b-negative/Isl1/2-positive somatic motor neurons, but not by Phox2b-positive/Isl1/2-positive branchial and visceral motor neurons, suggesting that Nolz-1 may regulate development of somatic motor neurons in the hindbrain. In addition to be expressed in differentiating post-mitotic neurons, Nolz-1 was also expressed by progenitor cells in the ventricular zone located in the dorsal part of aqueduct and the alar plates of hindbrain, which suggests a regulatory role of Nolz-1 in the germinal zone. Taken together, based on its domain- and cell type-selective pattern, Nolz-1 may involve in regulation of various developmental processes, including regional patterning and cell-type specification and differentiation in the developing mouse hindbrain. |
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Keywords: | Abbreviations: IV fourth ventricle BA branchial arches BM branchial motor neurons Cb cerebellum CLi caudal linear nucleus raphe DC dorsal cochlear nucleus dmnX dorsal motor nucleus of the vagus nerve DPGi dorsal paragigantocellular nucleus DTg dorsal tegmental nucleus fl forelimb Gi gigantocellular nucleus GiV ventral gigantocellular nucleus hl hindlimb ht heart primordium IOm medial nucleus of inferior olive IOpr principal nucleus of inferior olive IRt intermediate reticular zone ISH in situ hybridization LC locus coeruleus LDTg laterodorsal tegmental nucleus LPGi lateral paragigantocelullar nucleus MB (mb) midbrain MdD dorsal medullary reticular nucleus Me5 mesencephalic trigeminal nucleus MHB midbrain-hindbrain boundary nV trigerminal cranial nerve nXIl 12th cranial nerve op optic vesicles ot otic vesicles PCRt parvocellular reticular nucleus po pons rmed rostral medulla RVL rostroventrolateral reticular nucleus PrH prepostitus hypoglossal nucleus area s somites sc spinal cord SC superior coliculus SM somatic motor neurons Sp5 spinal trigeminal nucleus Sol nucleus of solitary tract ST striatal primordium SVZ subventricular zone VC ventral cochlear nucleus Ve vesticular nucleus VM visceral motor neurons VZ ventricular zone |
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