Demethylamination of N6-methyldeoxyadenosine by commercial spleen phosphodiesterase preparations |
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Authors: | A Dugaiczyk H W Boyer H M Goodman |
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Affiliation: | Departments of Biochemistry and Biophysics, and Microbiology University of California Medical Center San Francisco, California 94143 USA |
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Abstract: | Commercial spleen phosphodiesterase preparations contain an activity (presumably enzymatic) that will deaminate adenosine, deoxyadenosine and demethylaminate N6-methyldeoxyadenosine but not their 3′ -or 5′-phosphates. The product obtained by the enzymic demethylamination of N6-methyldeoxyadenosine was identified as deoxyinosine on the basis of its electrophoretic mobility and its ultraviolet spectrum. The presence of a deaminase activity in spleen phosphodiesterase preparations must be considered when using the enzyme in nucleotide sequence studies. |
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