Endophytic fungi from leaves of Centella asiatica: occurrence and potential interactions within leaves |
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| Authors: | E. F. Rakotoniriana F. Munaut C. Decock D. Randriamampionona M. Andriambololoniaina T. Rakotomalala E. J. Rakotonirina C. Rabemanantsoa K. Cheuk S. U. Ratsimamanga J. Mahillon M. El-Jaziri J. Quetin-Leclercq A. M. Corbisier |
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| Affiliation: | (1) Institut Malgache de Recherches Appliquées (IMRA), BP3833, Avarabohitra Itaosy, Antananarivo (102), Madagascar;(2) Mycothèque de l’Université catholique de Louvain (BCCM/MUCL), Unité de Microbiologie, Faculté d’Ingénierie biologique, agronomique et environnementale, Université catholique de Louvain, Croix du Sud 3 box 6, Louvain-la-Neuve, 1348, Belgium;(3) Institut National de Santé Publique et Communautaire, Service de Recherches et Expertise, Antananarivo (101), Madagascar;(4) Laboratory of Food and Environmental Microbiology, Unité de Microbiologie, Faculté d’Ingénierie biologique, agronomique et environnementale, Université catholique de Louvain, Croix du Sud 3 box 6, Louvain-la-Neuve, 1348, Belgium;(5) Laboratory of Plant Biotechnology, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium;(6) Laboratoire de Pharmacognosie, Unité CHAM, Université catholique de Louvain, Bruxelles, Belgium |
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| Abstract: | Fungal endophytes were isolated from leaves of Centella asiatica (Apiaceae) collected at Mangoro (middle eastern region of Madagascar, 200 km from Antananarivo). Forty- five different taxa were recovered. The overall foliar colonization rate was 78%. The most common endophytes were the non-sporulating species 1 (isolation frequency IF 19.2%) followed by Colletotrichum sp.1 (IF 13.2%), Guignardia sp. (IF 8.5%), Glomerella sp. (IF 7.7%), an unidentified ascomycete (IF 7.2%), the non-sporulating species 2 (IF 3.7%) and Phialophora sp. (IF 3.5%). Using sequences of the ribosomal DNA internal transcribed spacer (ITS) regions, major endophytes (IF > 7%) were identified as xylariaceous taxa or as Colletotrichum higginsianum, Guignardia mangiferae and Glomerella cingulata. Results from in vitro fungal disk experiments showed a strong inhibitory activity of the xylariaceous non-sporulating species 1 against G. mangiferae and C. higginsianum and of C. higginsianum against G. mangiferae. This can be explained by antagonism between dominant taxa. |
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| Keywords: | Centella asiatica Colletotrichum higginsianum Endophytic fungi Xylariaceae |
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