Mutants of translational components that alter reading frame by two steps forward or one step back |
| |
| Authors: | M B Falahee R B Weiss M O'Connor S Doonan R F Gesteland J F Atkins |
| |
| Affiliation: | Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132. |
| |
| Abstract: | External suppressors, sufS, of a -1 frameshift mutant cause ribosomes to shift into the -1 frame when reading the sequence CAG GGA GUG. The resulting product is not Gln-Gly-Val but Gln-Gly-Ser with Ser being encoded by the underlined AGU. The alleles investigated are approximately 2% efficient in causing frameshifting. Two other suppressors, hopR and hopE of the same -1 frameshift mutant, cause some ribosomes reading the sequence GUG UG to decode a single amino acid, Val, from the five nucleotides. The possibility is considered that peptidyl-tRNA(Val) dissociates from the mRNA, but re-pairs in a triplet manner after the mRNA slips forward by two bases. |
| |
| Keywords: | |
|
|
