Microbial transformation of immunosuppressive compounds III. Glucosylation of immunomycin (FR 900520) and FK 506 byBacillus subtilis ATCC 55060 |
| |
Authors: | Brain R Petuch Byron Arison Annjia Hsu Richard Monaghan Francis J Dumont Tom S Chen PhD |
| |
Institution: | (1) Fermentation Microbiology, Merck Research Laboratories, Building R80Y-205, PO Box 2000, 07065 Rahway, NJ, USA |
| |
Abstract: | Summary The regiospecific glucosylation of FK 506 and immunomycin (FR 900520) at the 24-hydroxy position was performed using resting cells ofBacillus subtilis ATCC 55060. 24-Glucopyranosyl FK 506 and 24-glucopyranosyl immunomycin were isolated by methylene chloride extraction and purification using reverse phase HPLC. The metabolite structures were established using spectroscopic techniques including MS and NMR. The glucose conjugate was further confirmed by chemical degradation. Enzymatic glucosylation was demonstrated using cell-free extracts derived fromBacillus subtilis ATCC 55060. The 24-glucosyltransferase, which appears UDP-glucose dependent, was solubilized from cell membranes by treatment with 0.1% Nonidet P-40 detergent. The optimal conditions for assay of the enzyme have been determined. |
| |
Keywords: | Microbial transformation FK 506 Bacillus subtilis |
本文献已被 SpringerLink 等数据库收录! |