Photophysical characterization of a FRET system using tailor-made DNA oligonucleotide sequences |
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Authors: | Flehr Roman Kienzler Andrea Bannwarth Willi Kumke Michael U |
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Affiliation: | University of Potsdam, Institute of Chemistry (Physical Chemistry), 14476 Potsdam-Golm, Germany. |
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Abstract: | We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation. |
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