| Abstract: | A series of hybrid clones have been isolated following the somatic cell fusion of two mammalian cell lines, each defective in junctional transfer of metabolites. One of these parental lines is a variant isolated by selection from the metabolic co-operation competent embryonal carcinoma line PC13TG8. The other parent is LMTK− in which inability to transfer was found to be a pre-existing property. Hybrids between these two cell lines are restored in their ability to co-operate, indicating the existence of at least two genetically distinct lesions affecting metabolic co-operation, each of which is recessive. This is the first demonstration that more than one locus is involved in junctional communication. |
