Limitations in substrate utilization efficiency by Zymomonas mobilis |
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Authors: | Sue Cromie Horst W Doelle |
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Institution: | (1) Department of Microbiology, University of Queensland, 4067 St. Lucia, Qld., Australia |
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Abstract: | Summary Optimal growth conditions for Zymomonas mobilis have been established using continuous cultivation methods. Optimal substrate utilization efficiency occurs with 2.5 g l–1 yeast extract, 2.0 g l–1 ammonium sulfate and 6.0 g l–1 magnesium sulfate in the media. Catabolic activity is at its maximum with glucose uptake rates of 16–18 g l–1 h–1 and ethanol production rates of 8–9 g l–1 h–1, Qg values of 22–26 and Qp values between 11 and 13, which results in 40 g l–1 h–1 ethanol yields using a 100 g l–1 substrate feed. Any increase in these parameters goes on cost of substrate utilization efficiency. Calcium pantothenate can not substitute yeast extract.Abbreviations G
Glucose (%)
- Pant
Calcium pantothenate (mg l–1)
- D
Dilution rate (h–1)
- NH4
Ammonium sulfate (%)
- Mg
Magnesium sulfate (%)
- S1
Residual glucose in the fermenter (g l–1)
- S0
Glucose feed (g l–1)
- Eth
Ethanol concentration (g l–1)
- GUR
Glucose uptake rate (g l–1 h–1)
- Qg
Specific glucose uptake rate (g g–1 h–1)
- Qp
Specific ethanol production rate (g g–1 h–1)
- EPR
Ethanol production rate (g l–1 h–1)
- Yg
Yield coefficient for glucose (g g–1)
- Yp
Conversion efficiency (%)
- C
Biomass concentration (g l–1)
Present address: (Until June 1982) Institut für Mikrobiologie, TH Darmstadt, 6100 Darmstdt, Federal Republic of Germany |
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