Interaction of Estrogen Receptor Associated Protein (ERAP) 140 with ER β Decreases but its Expression Increases in Aging Mouse Cerebral Cortex |
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Authors: | Vijay Paramanik M K Thakur |
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Institution: | (1) Biochemistry and Molecular Biology Laboratory, Centre of Advanced study, Department of Zoology, Banaras Hindu University, Varanasi, 221 005, India; |
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Abstract: | Following binding to cognate ligand, estrogen receptor (ER) β interacts with specific responsive elements of the target genes
and recruits a host of nuclear proteins for hormone dependent gene regulation. However, it is poorly known which proteins
interact with ER β in mouse brain and whether their interaction and expression change with age. In this report, we have used
his-tag mouse ER β for interaction with nuclear proteins of cerebral cortex of young (6 ± 1 weeks), adult (25 ± 2 weeks),
and old (70 ± 5 weeks) female mice. We have identified estrogen receptor-associated protein (ERAP) 140 as one of the interacting
proteins and studied its interaction by pull down immunoblotting, far-Western blotting and immunoprecipitation, and expression
by western blotting. The data show that ERAP 140 interacts with ER β and its interaction decreases but its expression increases
with age in mouse cerebral cortex, suggesting its role in estrogen-mediated brain functions during aging. |
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