Proteome analysis of haptoglobin in cerebrospinal fluid of neuromyelitis optica |
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Authors: | Shumei Bai Shilian Liu Xuxiao Guo Zhaoyu Qin Banqin Wang Xiaohong Li Yanjiang Qin |
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Institution: | (1) Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44 Wenhuaxi Road, 250012 Jinan, Shandong, People’s Republic of China;(2) Department of Clinical Laboratory, The Affiliated Hospital of Shandong University of Traditional Chinese Medical, 250014 Jinan, Shandong, People’s Republic of China;(3) Biology Science Department in Marine College, Shandong University at Weihai, 264209 Weihai, Shandong, People’s Republic of China;(4) Department of Neurology, Qilu Hospital of Shandong University, 250012 Jinan, Shandong, People’s Republic of China; |
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Abstract: | Neuromyelitis optica (NMO) is an inflammatory demyelinating disease with generally poor prognosis that selectively targets
optic nerves and spinal cord. Although diagnostic criteria for NMO are available, there is still a need for biomarkers, predicting
disease development and progression to improve individually tailored treatment. CSF proteins were separated by two-dimensional
electrophoresis and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF
MS). The interaction between these proteins was further analyzed by Pathway Studio software. Seven protein spots in CSF were
significantly altered in NMO patients compared with controls. Identification made by mass spectrometry revealed that the most
significant protein was haptoglobin, which was increased in the NMO gels. The subsequent ELISA test were performed to validate
it, which confirmed the results of proteomic analysis. Protein network was built, which showed some biological interactions
among the seven proteins. These results support a correlation between the level of haptoglobin and NMO. Haptoglobin may be
a potential useful biomarker for diagnosis or a medicine target for treatment of NMO. |
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