A colicin A fragment containing the receptor binding domain can be directed to the periplasmic space in E. coli through gene fusion |
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Authors: | V Crozel C Lazdunski R Lloubes D Cavard |
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Institution: | Centre de Biochimie et de Biologie Moléculaire du CNRS, 31 Chemin J. Aiguier BP 71, 13402 Marseille Cédex 9, France |
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Abstract: | The central region of the colicin A polypeptide chain has been fused to the N-terminal part of beta-lactamase through genetic recombination. This region comprising amino acid residues 70-335 confers on the hybrid protein the ability to protect sensitive cells from the lethal action of colicin A. Although colicin A belongs to the cytoplasmic compartment of E. coli, export of the hybrid protein to the periplasmic space was promoted by the signal peptide of beta-lactamase. |
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Keywords: | Colicin Export Receptor Hybrid protein |
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