Isolation and identification of prostaglandin PGB1 in growth cartilage

Prostaglandin PGB₁, and lesser amounts of other prostaglandin-like compounds, have been isolated from calf growth-cartilage by a series of chromatographic procedures. The major compound, present at about 3 ppm fresh weight, was identified as PGB₁ by comigration with authentic PGB₁ in a wide variety of thin-layer chromatographic solvent systems; as well as by infrared and UV spectroscopy, and by radioimmunoassay. The presence in cartilage of larger amounts of PGB₁ than the other kinds of prostaglandins may be due to a conversion of PGE₁ to PGB₁ in vivo,or perhaps during the extraction and isolation procedures.