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Crystalline monoclonal antibody Fabs complexed to hen egg white lysozyme |
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| Authors: | E W Silverton E A Padlan D R Davies S Smith-Gill M Potter |
| Affiliation: | The Laboratory of Molecular Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health Bethesda, Md 20205, U.S.A.;The Laboratory of Genetics National Cancer Institutes of Health Bethesda, Md 20205, U.S.A. |
| Abstract: | The Fab of a monoclonal anti-lysozyme antibody (HyHEL-10) has been crystallized as the free Fab and as the Fab-antigen complex. Crystals have also been grown of the antigen complex of the Fab of another monoclonal anti-lysozyme antibody (HyHEL-9), which recognizes a different binding surface of lysozyme. All three crystals diffract to at least 3 A resolution and are suitable for X-ray diffraction studies. |
| Keywords: | HEL hen egg white lysozyme HyHEL hybridoma antibody against hen egg white lysozyme IgG immunoglobulin G PEG polyethylene glycol MPD 2-methyl,2,4-pentanediol |
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