Purine nucleoside phosphorylase (Np) in the mouse: Linkage relationship of Np-2 to esterase-10 (Es-10) and Np-1 on chromosome 14 |
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| Authors: | Trevor Lukey Kuldeep Neote John F. Loman Ardythe E. Unger Fred G. Biddle Floyd F. Snyder |
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| Affiliation: | (1) Departments of Pediatrics and Medical Biochemistry, Faculty of Medicine, The University of Calgary, T2N 4N1 Calgary, Alberta, Canada |
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| Abstract: | An improved method for detecting four Np-1 (purine nucleoside phosphorylase) alleles in mouse erythrocytes by cellulose acetate electrophoresis is described. The previous linkage of Np-1 and Es-10 (esterase-10) was confirmed, with a map distance of 13.0±2.6 cM. Np-2 was detected by either specific activity assay or starch gel electrophoresis and shown to be linked to Es-10, 15.9 ± 3.1 cM, on chromosome 14. No recombinants between Np-1 and Np-2 were observed in 52 offspring, indicating either that these loci are either closely associated or that Np-2 represents simply a property of existing allelic products of the Np-1 locus.This research was supported by Medical Research Council of Canada grants to F.G.B. and F.F.S. |
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| Keywords: | mouse nucleoside phorphorylases 1 and 2 esterase-10 chromosome 14 |
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