Agrobacterium-mediated transformation and regeneration of kiwi fruit |
| |
Authors: | Chiyomi Uematsu Makoto Murase Hiroaki Ichikawa Jun Imamura |
| |
Affiliation: | (1) Plantech Research Institute, Kamoshida-cho 1000, Midoriku, 227 Yokohama, Japan;(2) Present address: Botanical Gardens, Faculty of Science, Osaka City University, Kisaichi 2000, Katano, 576 Osaka, Japan |
| |
Abstract: | Summary Genetically transformed kiwi fruit (Actinidia deliciosa) plants were obtained from hypocotyl and stem segments co-cultured with Agrobacterium tumefaciens strain EHA101 harboring a binary vector, pLAN411 or pLAN421, which contained the neomycin phosphotransferase II (nptII) gene and the -glucuronidase (GUS) gene. After co-culturing with the A. tumefaciens, the hypocotyl or stem segments were cultured on a selection medium containing 25 g/ml kanamycin and 500 g/ml Claforan. After one month in culture, shoots had regenerated from the cuttings. Green shoots were analyzed for NPTII activity and GUS activity. Eighty-five percent of the green shoots examined expressed the nptII and GUS genes. GUS histochemical assays revealed strong GUS expression in guard cells, mesophyll cells, and trichomes. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|