|
|||||
|
|
Gamma-endorphin-like peptides in pituitary tissue: evidence for their existence in vivo |
|
| Authors: | D M Dorsa M B Chapman D G Baskin |
| Affiliation: | 1. Geriatric Research, Education and Clinical Center, Seattle, WA 98108 USA;2. Division of Endocrinology/Metabolism VA Medical Center, 4435 Beacon Avenue South, Seattle, WA 98108 USA;3. Department of Medicine, University of Washington, Seattle, WA USA;4. Department of Pharmacology, University of Washington, Seattle, WA USA;5. Department of Biological Structure University of Washington, Seattle, WA USA |
| Abstract: | Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both beta E- and gamma E-like peptides. The gamma E-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic gamma E upon gel permeation chromatography. Immunoreactivity for beta E-like and gamma E-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that gamma E-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue. |
| Keywords: | γ-Endorphin β-Endorphin Pituitary Radioimmunoassay Immunocytochemistry |
| 本文献已被 ScienceDirect 等数据库收录！ | |