| 普通小麦抗、感赤霉病品种(系)的RAPD标记分析 |
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| 引用本文： | 张小红 陈耀锋 任惠莉 闵东红. 普通小麦抗、感赤霉病品种(系)的RAPD标记分析[J]. 西北植物学报, 2005, 25(5): 893-897 |
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| 作者姓名： | 张小红 陈耀锋 任惠莉 闵东红 |
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| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西杨陵,712100
2. 西北农林科技大学,农学院,陕西杨陵,712100 |
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| 基金项目： | 陕西省分子生物学重点实验室项目 |
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| 摘 要： | 采用RAPD技术，用524个随机引物对3个抗病品种和4个感病品种的基因组DNA进行扩增，发现3个引物能在抗、感品种间检测到稳定的多态性。这3个引物及它们产生的多态片段是S347 1220bp、S372 872bp和S375 1360bp，其中S347 1220bp和S372 875bp在感病品种上呈显性扩增，在抗病品种上无扩增，相反S375 1360bp在感病品种上无扩增，在抗病品种上呈显性扩增。初步判断这3个特异带与小麦赤霉病抗性有关。
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| 关 键 词： | 小麦 赤霉病 RAPD |
| 文章编号： | 1000-4025(2005)05-0893-05 |
RAPD Marker Analysis of Scab-resistant and -Susceptible Varieties (Lines) of Common Wheat |
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| ZHANG Xiao-hong,CHEN Yao-feng. RAPD Marker Analysis of Scab-resistant and -Susceptible Varieties (Lines) of Common Wheat[J]. Acta Botanica Boreali-Occidentalia Sinica, 2005, 25(5): 893-897 |
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| Authors: | ZHANG Xiao-hong CHEN Yao-feng |
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| Affiliation: | ZHANG Xiao-hong~1,CHEN Yao-feng~ |
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| Abstract: | 524 random primers were used in RAPD analysis to amplify genomes of three scab-resistant varieties and four scab-susceptible varieties and three of them were found to show stable polymorphism in both the scab-resistant and -susceptible varieties.The polymorphic bands amplified with the three primers were S347_(1 220 bp),S372_(875 bp) and S375_(1 360 bp).Of the bands,S347_(1 220 bp) and S372_(875 bp) were found in all the susceptible varieties but not in all the resistant varieties whereas S375_(1 360 bp) was not found in all the susceptible varieties but found in all the resistant varieties. It was preliminarily concluded that these three specific bands related to scab resistance. |
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| Keywords: | wheat scab RAPD |
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