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Signal Peptide of Potato PinII Enhances the Expression of Cry1Ac in Transgenic Tobacco |
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| Authors: | Yun-Jun LIU Yuan YUAN Jun ZHENG Ya-Zhong TAO Zhi-Gang DONG Jian-Hua WANG and Guo-Ying WANGState Key Laboratory for Agrobiotechnology National Center for Maize Improvement China Agricultural University Beijing China |
| Affiliation: | Yun-Jun LIU,Yuan YUAN,Jun ZHENG,Ya-Zhong TAO,Zhi-Gang DONG,Jian-Hua WANG,and Guo-Ying WANGState Key Laboratory for Agrobiotechnology and National Center for Maize Improvement,China Agricultural University,Beijing 100094,China |
| Abstract: | The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants. |
| Keywords: | Cry1Ac signal peptide GFP transgenic tobacco |
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