Inducible long chain alcohol oxidase from alkane-grown Candida tropicalis

Summary The oxidation of primary aliphatic alcohols by microsomal membrane fractions of alkane grown Candida tropicalis was shown to be due to the action of an inducible alcohol oxidase with a wide substrate specificity towards aliphatic alcohols. Stoichiometric studies showed that NADH production, in the presence of fatty alcohols, was due to the activity of an inducible fatty aldehyde dehydrogenase. The oxidase activity could be measured directly by hydrogen peroxide production via a peroxidase and a chromogenic redox indicator.