Effect of freezing and thawing on cell membranes of Lentinus edodes,the shiitake mushroom |
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Authors: | M. F. Roquebert E. Bury |
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Affiliation: | (1) Museum National d'Histoire Naturelle, Laboratoire de Cryptogamie, 12 rue Buffon, 75005 Paris, France |
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Abstract: | When cell membranes of Lentinus edodes mycelium were rapidly frozen at either 50 or 160°C/min, viability was lost and this correlated with rupture of the plasmalemma and residual membrane material and with alterations in the organelles. Although with slow cooling (1°C/min) 80% of the samples recovered viability, some cells still showed similar changes to those cooled rapidly, indicating that individual cells of the mycelium do not respond in the same way. |
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Keywords: | Cryopreservation Lentinus edodes membranes ultrastructure |
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