Rhesus macaque MHC class I molecules show differential subcellular localizations |
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Authors: | Cornelia Rosner Philip H Kruse Torben Lübke Lutz Walter |
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Institution: | 1. Abteilung Primatengenetik, Deutsches Primatenzentrum—Leibniz Institut für Primatenforschung, Kellnerweg 4, 37077, G?ttingen, Germany 2. Institut für Biochemie 2, Universit?t G?ttingen, G?ttingen, Germany
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Abstract: | The MHC class I gene family of rhesus macaques is characterised by considerable gene duplications. While a HLA-C-orthologous gene is absent, the Mamu-A and in particular the Mamu-B genes have expanded, giving rise to plastic haplotypes with differential gene content. Although some of the rhesus macaque
MHC class I genes are known to be associated with susceptibility/resistance to infectious diseases, the functional significance of duplicated
Mamu-A and Mamu-B genes and the expression pattern of their encoded proteins are largely unknown. Here, we present data of the subcellular
localization of AcGFP-tagged Mamu-A and Mamu-B molecules. We found strong cell surface and low intracellular expression for
Mamu-A1, Mamu-A2 and Mamu-A3-encoded molecules as well as for Mamu-B*01704, Mamu-B*02101, Mamu-B*04801, Mamu-B*06002 and Mamu-B*13401. In contrast, weak
cell surface and strong intracellular expression was seen for Mamu-A4*1403, Mamu-B*01202, Mamu-B*02804, Mamu-B*03002, Mamu-B*05704,
Mamu-I*010201 and Mamu-I*0121. The different expression patterns were assigned to the antigen-binding α1 and α2 domains, suggesting
failure of peptide binding is responsible for retaining ‘intracellular’ Mamu class I molecules in the endoplasmic reticulum.
These findings indicate a diverse functional role of the duplicated rhesus macaque MHC class I genes. |
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