Studies on Correlation Between Single-Nucleotide Polymorphisms of Tumor Necrosis Factor Gene and Different Stages of Ankylosing Spondylitis |
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Authors: | Yinghua Ji Xiaoyu Yang Lin Yang Dapeng Wu Fangfang Hua Tan Lu Jinling Jia Chao Ma Qiudong Liang |
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Institution: | 1. Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Xinxiang, China 2. Department of Pathology, Xinxiang Medical University, Xinxiang, China 3. Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Xinxiang, China 4. The First Affiliated Hospital of Xinxiang Medical University, Weihui, XinXiang, China
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Abstract: | To examine if there is any correlation between ankylosing spondylitis (AS) and TNF-α gene promoter single-nucleotide polymorphisms (SNP) and their associated haplotypes. Using restriction fragment length polymorphism—polymerase chain reaction method, the polymorphism of TNF-α-238, -308, -850, -857, -863 locus, and TNF-β +252 were analyzed in patients with progressive AS, stable AS and control. (1) Neither the genotypes nor the allele frequencies of TNF-α (-308), (-238), (-863), and TNF-β +252 showed differences in each group. TNF-α (-850) CC genotype and C allele frequency distribution was significantly higher in healthy controls group than in the stable and progressive groups. TNF-α (-857) CT, CC genotype, and C, T allele frequency showed differences in all groups. (2) Polymorphism linkage equilibrium test revealed that association of six TNF-α, β gene SNPs with haplotype GACTCG in progressive group is significantly higher than in the stable group and healthy control group (P < 0.05). TNF-α (-857), (-850) gene polymorphism may increase the susceptibility to AS, but do not reflect the disease active state. The CC genotype and C allele may play a protective role in the pathogenesis of AS. TNF-α (-308) may be a weak indicator reflecting the active state of AS. Haplotype GACTCG may indicate both the susceptibility and the activity of AS. |
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