Localization of the HIV-1 gp120 Conformational Epitope Recognized by Virus-Neutralizing Monoclonal Antibodies 2G12 |
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| Authors: | Tumanova O. Yu. Kuvshinov V. N. Il'ichev A. A. Nekrasov B. G. Ivanisenko V. A. Kozlov A. P. Sandakhchiev L. S. |
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| Affiliation: | (1) VECTOR State Research Center of Virology and Biotechnology, Institute of Bioengineering, Kol'tsovo, Novosibirsk Region, 630559, Russia;(2) Biomedical Center, Institute of Highly Purified Preparations, St. Petersburg, 197110, Russia |
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| Abstract: | A phage peptide library was used to select peptides interacting with virus-neutralizing monoclonal antibodies (mAb) 2G12 which recognize a discontinuous surface epitope of HIV-1 gp120. With the published X-ray data, gp120 regions involved in the antigenic determinant were predicted. Binding with mAb 2G12 was ascribed to Thr297, Phe383, Tyr384, Arg419, Ile240, Thr415, Leu416, Pro417, Lys421, and Trp112. Though distant in the gp120 sequence, these residues are close in space and form the 2G12 epitope on the gp120 surface. |
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| Keywords: | HIV-1 gp120 virus-neutralizing monoclonal antibody 2G12 conformational epitope localization phage peptide library |
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