Immunocytochemical localization of H+?K+-ATPase in the rat colon |
| |
Authors: | Lukas Haragsim Bahar Bastani and Jian Ping Li |
| |
Institution: | (1) Division of Nephrology, St. Louis University Health Sciences Center, 3635 Vista Avenue, 63110 St. Louis, Missouri, USA |
| |
Abstract: | Summary The presence and distribution of gastric-type H+−K+-ATPase were investigated in the rat colon using a monoclonal antibody raised against hog gastric H+−K+-ATPase. Rat stomach was used as positive control. Rat kidney and ileum, in both of which H+−K+-ATPase has been reported in the past, were also studied.
In stomach, very strong staining was found confined to the parietal cells, and a strong band atM
r∼94 kDa on the immunoblots. In colon a moderate staining was found in the supranuclear region of the epithelial cells, with
similar intensity and distribution of staining of the surface and deep mucosa of the crypts, throughout the length of the
colon. Another monoclonal antibody, specific to the 31 kDa subunit of H+-ATPase, used as a negative control, or omission of the primary antibody, resulted in lack of any staining in either colon
or stomach. On immunoblots of homogenates of colonic mucosa, no specific band could be identified, either due to very low
expression of the H+−K+-ATPase or loss of antigenicity of the epitope during the processing steps. No positive staining was observed in rat kidney
and ileum, suggesting that they contain isoforms that are structurally different. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|