α-亚麻酸对糖尿病大鼠炎症介质和氧化应激的影响

目的:观察α-亚麻酸(ALA)对糖尿病大鼠体内炎症介质和氧化应激的影响,探讨ALA在糖尿病防治中的作用。方法:雄性SD大鼠高脂饮食喂养4周后,腹腔注射链脲佐菌素(STZ)30 mg/kg建立2型糖尿病(T2DM)模型。将大鼠随机分为3组(n=10):正常对照组、糖尿病模型组和ALA治疗组(500μg/kg.d)。4周后测定大鼠血清中肿瘤坏死因子(TNF-α)、可溶性P-选择素(sP-selectin)、可溶性细胞间黏附分子(sICAM-1)、一氧化氮(NO)、丙二醛(MDA)的含量以及超氧化物岐化酶(SOD)和过氧化氢酶(CAT)的活性。结果:与正常对照组相比,糖尿病大鼠血清中炎症介质TNF-α、sP-selectin和sICAM-1的含量增加,血清NO含量下降而MDA升高,同时抗氧化酶SOD和CAT的活性降低;ALA治疗可显著降低糖尿病大鼠血清中TNF-α、sP-selectin和sICAM-1的含量(与STZ+vehicle组相比,P<0.01),增加血清NO水平并减少MDA含量,升高抗氧化酶SOD和CAT的活性(与STZ+vehicle组相比,均P<0.05)。结论:ALA可显著降低糖尿病大鼠血清炎症介质的生成,减轻氧化应激水平,具有抗炎和抗氧化作用。提示ALA对糖尿病及糖尿病并发症的发生发展可能具有一定的防治作用。

Effects of alpha-linolenic acid on inflammation and oxidative stress in the diabetic rats

Objective: To investigate the effects of alpha-linolenic acid(ALA) on inflammation and oxidative stress in the diabetic rats.Methods: An experimental type 2 diabetes mellitus model was induced by feeding male SD rats with diet of high fat for 4 weeks and then injected them intraperitoneally with streptozocin(STZ) at 30 mg/kg.Then the animals were randomely divided into three groups(n=10): control group,diabetic group and ALA group.Four weeks later,tumor necrosis factor(TNF)-α,soluble P-selectin(sP-selectin),soluble intercellular adhesion molecule-1(sICAM-1),nitric oxide(NO) production,malondialdehyde(MDA),superoxide dismutase(SOD) and catalase(CAT) in the serum were determined.Results: Inflammatory agents including TNF-α,sP-selectin and sICAM-1 increased in diabetic rats to compare with control group.Treatment with ALA significantly decreased TNF-α,sP-selectin and sICAM-1 to compare with diabetic group.Furthermore,compared with control group,serum MDA production increased whereas NO production,SOD and CAT activities decreased in diabetic rats.Treatment with ALA reduced MDA production,increased NO production,promoted SOD and CAT activities compared with diabetic group.Conclusion: These results indicate that diet rich in ALA exerted the anti-inflammatory and anti-oxidative effects in diabetic rats,which may be beneficial to the prevention and treatment of diabetes.